Journal: PLoS ONE

Article Title: Attenuation of Vaccinia Tian Tan Strain by Removal of Viral TC7L-TK2L and TA35R Genes

doi: 10.1371/journal.pone.0031979

Figure Lengend Snippet: Effects of MVTT1, MVTT2, MVTT3, or VTT (0.05 PFU/cell) and infection times on the cell viabilities of HeLa (A), MDCK (B), PK(15) (C), BHK-21 (D), and Vero (E). Cells were seeded in 96-well plates (1×10 4 cells/well) one day before they were infected with 0.05 PFU/cell of MVTT1, MVTT2, MVTT3, or VTT. Cell viability was measured daily for 4 d by MTT colorimetric assay. All measurements were performed in triplicate. Data are presented as mean ± SD.

Article Snippet: BHK-21 hamster kidney cells, PK(15) porcine kidney cells, HeLa human cervical adenocarcinoma epithelial cells, Madin-Darby canine kidney (MDCK) epithelial cells, and Vero African green monkey kidney cells were obtained from the China Center for Type Culture Collection.

Techniques: Infection, Colorimetric Assay

Journal: PLoS ONE

Article Title: Attenuation of Vaccinia Tian Tan Strain by Removal of Viral TC7L-TK2L and TA35R Genes

doi: 10.1371/journal.pone.0031979

Figure Lengend Snippet: Confluent monolayers of PK(15), MDCK, HeLa, BHK-21 and Vero cells in 12-well plates were infected with 0.05 PFU/cell of MVTT1, MVTT2, MVTT3, or VTT viruses. The plates were incubated at 37°C for 2 d prior to staining with 0.1% crystal violet. The pathogenicity of MVTT1, MVTT2, and MVTT3 apparently decreased in all five cell lines (compared with VTT as the control).

Article Snippet: BHK-21 hamster kidney cells, PK(15) porcine kidney cells, HeLa human cervical adenocarcinoma epithelial cells, Madin-Darby canine kidney (MDCK) epithelial cells, and Vero African green monkey kidney cells were obtained from the China Center for Type Culture Collection.

Techniques: Infection, Incubation, Staining, Control

Journal: PLoS ONE

Article Title: Attenuation of Vaccinia Tian Tan Strain by Removal of Viral TC7L-TK2L and TA35R Genes

doi: 10.1371/journal.pone.0031979

Figure Lengend Snippet: PK(15), MDCK, HeLa, BHK-21 and Vero cells infected with 0.05 MOI of VTT and the mutants, and then the viruses were harvested and titrated in BHK-21 cells. Virus titer was determined by measuring the plaque assays.

Article Snippet: BHK-21 hamster kidney cells, PK(15) porcine kidney cells, HeLa human cervical adenocarcinoma epithelial cells, Madin-Darby canine kidney (MDCK) epithelial cells, and Vero African green monkey kidney cells were obtained from the China Center for Type Culture Collection.

Techniques: Infection, Virus

Journal: Antioxidants

Article Title: In Vitro α-Glucosidase and α-Amylase Inhibition, Cytotoxicity and Free Radical Scavenging Profiling of the 6-Halogeno and Mixed 6,8-Dihalogenated 2-Aryl-4-methyl-1,2-dihydroquinazoline 3-Oxides

doi: 10.3390/antiox12111971

Figure Lengend Snippet: IC 50 values of 3a – p against α-glucosidase, α-amylase, MCF-7, A549 and HEK293-T.

Article Snippet: Cytotoxicity was measured in breast cancer (MCF-7), lung cancer (A549) and the HEK293-T cell lines (Cellonex Separation Scientific SA (Pty) Ltd. (Roodepoort, Johannesburg, South Africa) using the standard 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay [ ] against doxorubicin and gefitinib as positive controls.

Techniques:

Journal: Scientific Reports

Article Title: ROCK1/MLC2 inhibition induces decay of viral mRNA in BPXV infected cells

doi: 10.1038/s41598-022-21610-9

Figure Lengend Snippet: ROCK1 inhibition impairs BPXV-induced cell contraction and viral protein synthesis. ( a ) Effect of Thiazovivin on MLC2 phosphorylation (activation). Vero cells were either mock-infected or infected with BPXV at MOI of 5. Thiazovivin or vehicle controls were added at 4 hpi. Cell lysates were prepared at 9 hpi and subjected for detection of the p-MLC2 levels in Western blot analysis (ai). The histogram (aii) shows the band intensity of the protein. The blots were quantified by densitometry (ImageJ) and the data are presented as mean with SD. n = 3 independent experiments. See Supplementary Fig. for full blots. ( b ) Effect of Thiazovivin on BPXV induced cell contraction and levels of viral proteins. HeLa cells were grown in chamber slides and infected with BPXV at an MOI of 5 for 1 h. Thiazovivin was applied at 4 hpi. At 15 hpi, BPXV (FITC) proteins were probed by immunofluorescence assay. Cell morphology and level of viral proteins of Thiazovivin-treated or untreated cells is shown (bi). The histogram shows the relative reduction in cell size (bii) and relative levels of BPXV proteins (biii) in Thiazovivin treated or untreated cells. The area (n = 50 cells) and the intensity of viral proteins (n = 50 cells) were quantified by ImageJ. The data are presented as mean with SD. Pair-wise statistical comparisons were performed using Student's t-test (***P < 0.001; **P < 0.001).

Article Snippet: African green monkey kidney (Vero) and HeLa cells were received from National Centre for Cell Science (NCCS), Pune, India.

Techniques: Inhibition, Phospho-proteomics, Activation Assay, Infection, Western Blot, Immunofluorescence